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• 18:56, 20 October 2022 James talk contribs created page PHRAP-PHRED (Created page with "PHRAP is a program for shotgun sequence assembly, but it can also be used for small sequence assemblies. Its key features include its use of data quality information, both direct (from phred trace analysis) and indirect (from pairwise read comparisons), to delineate the likely accurate base calls in each read. This helps discriminate repeats. It permits the use of the full reads in assembly, and allows a highly accurate consensus sequence to be generated. A probability...")